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KMID : 1159320140160010001
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Korean Journal of Vision Science
2014 Volume.16 No. 1 p.1 ~ p.9
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A comparison of antibacterial effect of RGP lens multi-purpose solutions against Staphylococcus aureus and Pseudomonas aeruginosa
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Na Hun-Taek
Kim Hong-Seon
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Abstract
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Purpose: The compare each time antibacterial effects after soaking on multi-purpose solutions using Staphylococcus aureus and Pseudomonas aeruginosa contaminated RGP lens, and suggest the soaking time required for disinfecting contact lenses.
Methods: Culture media for Staphylococcus aureus and Pseudomonas aeruginosa (concentrat ion of 10 Colony Forming Unit/ml) 0.1ml and in LB broth 0.9ml mixture after inserting on RGP lens were performed after 24 hour of incubation. we compared the antibacterial effect of three different multi-purpose solutions with 1 hour soaking, 2 hours soaking, 3 hours soaking, 4 hours soaking, 5 hours soaking, 6 hours soaking, and 7 hours soaking.
Results: Soaking in multi-purpose solutions for 1 hour reduced the number of Staphylococcus aureus and Pseudomonas aeruginosa up to 43~47%, 55~63%. soaking in multi-purpose solutions for 2 hour reduced the number of Staphylococcus aureus and Pseudomonas aeruginosa up to 77~80%, 77~86%. soaking in multi-purpose solutions for 3 hour reduced the number of Staphylococcus aureus and Pseudomonas aeruginosa up to 97~98%, 85~97%. soaking in multi-purpose solutions for 4 hour reduced the number of Staphylococcus aureus and Pseudomonas aeruginosa up to 100%, 95~100%. Staphylococcus aureus and Pseudomonas aeruginosa was not detected at RGP lens soaked for 5 hour, 6 hour, 7 hour.
Conclusions: Staphylococcus aureus was not detected at soaking time for more than 4 hours multi-purpose solutions, Pseudomonas aeruginosa was not detected at soaking time for more than 5 hours multi-purpose solutions. Therefore, when you used disinfecting RGP contact lens for multi-purpose solutions used in this study should be that over 5 hours soaked.
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KEYWORD
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RGP lens multipurpose solutions, Staphylococcus aureus, Pseudomonas aeruginosa, antibacterial effect
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